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dc.contributor.advisor Huang, Dr. Patrick
dc.contributor.advisor Kim, Dr. Chul
dc.creator Sigua, Levi Martin
dc.date 2017-09-01
dc.date.accessioned 2017-09-20T23:21:57Z
dc.date.available 2017-09-20T23:21:57Z
dc.date.issued 2017-09-20
dc.identifier.uri http://hdl.handle.net/10211.3/196233 en
dc.description.abstract Galactonate-binding aptamers were isolated using a technique called Systematic Evolution of Ligands by Exponential Enrichment (SELEX). This iterative process was able to remove unwanted aptamers from a pool of oligonucleotide library. The target molecule (galactonate-Shinkai complex) was eventually reduced to 250 and 10 μM after several rounds of SELEX and observing selectivity. During counter-selection however, it showed that the aptamers were heavily binding to 100 μM Shinkai. Additional SELEX rounds were applied to further enrich the DNA pool. Insertion of the isolated DNAs in E. coli did not provide enough bacterial colonies for DNA sequencing. A 20-step synthetic route was proposed to synthesize two cryptophane molecules. The cryptophane-cage molecule is able to bind with 129Xe which is used in contrast agents to improve signal detection. Precursors for a cryptophane cage molecule were successfully synthesized and characterized. Additional works are needed to complete the proposed steps. en_US
dc.language English en_US
dc.subject Aptamers en_US
dc.title Isolation of Aptamers for Galactonate and Synthesis of Cryptophane Derivative Precursors en_US
dc.type Thesis en_US
dc.contributor.primaryAdvisor Halim, Dr. Marlin
thesis.degree.name Master of Science in Chemistry en_US

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