A partial characterization of the Pisolithus tinctorius microbiome

Relationships between the three domains of life can provide insight in the origins of life on Earth and the evolution of Eukaryotes by symbiogenesis and horizontal gene transfer. Archaeal and Bacterial interactions and possible contributions with Eukarya continue to be identified. The microbiome of a Yellowstone National Park geothermal pool proximal fungus, Pisolithus tinctorius, was partially characterized using standard microbiological approaches and metagenomic analysis. Archaeal and bacterial genera were identified by Next Generation Sequencing and De Novo and Reference-based assembly. Liquid Chromatography-Mass Spectrometry identified compounds present in Archaea and Bacteria. Identification of the microbiome community provides information regarding symbiotic associations.